Anti-Human IgM Fc5µ was produced by repeated immunization with Human IgM Fc5µ fragment in mice.
Konjugation:
Unconjugated
Alternative Synonym:
mouse anti-Human IgM Fc5µ Antibody, Human IgM Fc5u Antibody, Ms-a-Human IgM Fc5µ Antibody, Human IgM Fc5µ Antibody in Mouse, Human M Fc5µ Secondary Antibody
Format:
IgG2b
Spezifität:
IgM (µ)
Minimale Kreuzreaktivität (MinX):
no cross-adsorbtion
Klonalität:
Monoclonal
Konzentration:
1.0 mg/ml by UV absorbance at 280 nm
Klon-Bezeichnung:
[22G1.H8]
Isotyp:
IgG2b
Puffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung:
Liquid (sterile filtered)
Target-Kategorie:
Human
Antibody Type:
Secondary Antibody
Application Verdünnung:
ELISA: 1:1000 - 1:10000, WB: 1:1000-1:2000
Anwendungsbeschreibung:
Human IgM Fc5µ antibody has been tested by ELISA and Western Blot. This product is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specif
Western Blot of Mouse anti-Human IgM Fc5µ antibody. Lane 1: Human IgM (p/n 009-0107). Lane 2: Human IgG (p/n 009-0102). Lane 3: Human Fc (p/n 009-0103). Lane 4: Human Fab (p/n 009-0105). Lane 5: Human Fab2 (p/n 009-0104). Lane 6: Human IgM Fc5µ (p/n 009-0131). Load: 50 ng whole molecule per lane. Primary antibody: anti-Human IgM Fc5µ monoclonal antibody at 1:1000 for overnight at 4 C. Secondary antibody: Peroxidase anti-mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicated/Observed size: 78 kDa, 78 kDa for Human IgM.
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