Rabbit IgM (mu) Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal

Artikelnummer: ROC-211-1307
Artikelname: Rabbit IgM (mu) Antibody Peroxidase Conjugated, HRP, Goat, Polyclonal
Artikelnummer: ROC-211-1307
Hersteller Artikelnummer: 211-1307
Alternativnummer: ROC-211-1307
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA
Spezies Reaktivität: Rabbit
Immunogen: Anti-Rabbit IgM (mu) was produced by repeated immunization with rabbit IgM mu heavy chain in goat.
Konjugation: HRP
Alternative Synonym: Rabbit IgM (mu) Antibody, Gt-a-Rabbit IgM (mu) Peroxidase conjugated, Rabbit IgM (mu) Peroxidase Antibody in goat, Goat peroxidase conjugated Secondary Antibody, goat anti-rabbit IgM (mu chain) HRP conjugated antibody
Format: IgG
Spezifität: IgM (µ)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 10.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Rabbit
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:20,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
Anwendungsbeschreibung: Rabbit IgM (mu) peroxidase conjugated antibody has been tested by ELISA and is assayed against 1.0 ug of Rabbit IgM in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minut
ELISA Results of Goat Anti-Rabbit IgM Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Rabbit IgM (p/n 011-0107). The working dilution is 39,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB substrate (p/n TMBE-1000).