Goat IgG anti-Chicken IgM (µ)-unconj., MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Chicken IgM (µ)-unconj., MinX none
• Artikelnummer: ROC-603-101-007
• Hersteller Artikelnummer: 603-101-007
• Alternativnummer: ROC-603-101-007
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Gallus
• Immunogen: Chicken IgM whole molecule
• Konjugation: Unconjugated
• Alternative Synonym: goat anti-Chicken IgM (mu chain) Antibody, goat anti-Chicken IgM mu chain Antibody
• Format: IgG
• Spezifität: IgM (µ)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.1 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Chicken
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Chicken IgM antibody has been tested by ELISA and is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

Bilder

SDS-Page of Goat anti-Chicken IgM (Mu Chain) antibody. Lane 1: Gt-a-Chicken IgM Antibody - Non-Reduced. Lane 2: Gt-a-Chicken IgM Antibody - Reduced. Load: 1.0 µg per lane. Predicted/Observed size: 50 kDa, 25 kDa for Reduced. Other band(s): none.

Western Blot of Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG F(ab) Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated (p/n 603-103-007) 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.