Goat IgG anti-Chicken IgG (H+L)-Alk. Phos., MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Goat IgG anti-Chicken IgG (H+L)-Alk. Phos., MinX Bo,Go,Gp,Hm,Ho,Hu,Ms,Rb,Rt,Sh, ALP, Polyclonal , AP
• Artikelnummer: ROC-603-105-126
• Hersteller Artikelnummer: 603-105-126
• Alternativnummer: ROC-603-105-126
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Gallus
• Immunogen: Anti-Chicken IgG whole molecule was produced by repeated immunization with Chicken IgG whole molecule in goat.
• Konjugation: Alk. Phos.
• Alternative Synonym: Alk Phos Chicken Antibody, Gt-a-Chicken IgG Alkaline Phosphatase, Chicken Antibody in Goat, Chicken Secondary Antibody, Alk Phos Gt anti-Ch IgY Antibody, Goat Anti-Chicken IgY Alkaline Phosphatase,
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Bovine,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rabbit,Rat,Sheep

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Chicken
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Chicken IgG Alkaline Phosphatase Conjugate has been tested by ELISA, dot blot, and western blot. This product is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditio

Bilder

Western Blot of Unconjugated Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-101-126). Lane M: 3 µl Molecular Ladder. Lane 1: Chicken IgG/IgY whole molecule (p/n 003-0102). Lane 2: Chicken IgG F(c) Fragment (p/n 003-0103). Lane 3: Chicken IgG Fab Fragment (p/n 003-0105). Lane 4: Chicken IgM Whole Molecule (p/n 003-0107). Lane 5: Chicken Serum (p/n D302-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Chicken IgG (H&L) (GOAT) Antibody (Min X Bv Gt GP Ham Hs Hu Ms Rb Rt & Sh Serum Proteins) (p/n 603-101-126) 1:3,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgY and Serum, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher.

Western Blot of Alkaline Phosphatase Conjugated Goat anti-Chicken IgG Pre-Adsorbed secondary antibody. Lane 1: Chicken IgG/IgY (p/n 003-0103). Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Alkaline Phosphatase goat secondary antibody (603-105-126) at 1:1,000 for 60 min at RT. Block: (p/n MB-070) for 30 min at RT. Predicted/Observed size: 25 & 72 kDa, 25 & 72 kDa for Chicken IgY. Other band(s): None.