Anti-Goat IgG Pre-Adsorbed Antibody Peroxidase conjugated has been tested by ELISA and is suitable for western blot or dot blot, ELISA and immunohistochemistry, immunoperoxidase electron microscopy and as well as other enzymatic assays requiring lot-to-l
ELISA Results of Rabbit Anti-Goat IgG mx3 Antibody Peroxidase Conjugated. Each well was coated in duplicate with 10 µg of Goat IgG (p/n 005-0102). The working dilution of Goat IgG mx3 HRP is 1:91,000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer (p/n MB-066), Goat IgG mx3 HRP conjugated and TMB substrate (p/n TMBE-1000).
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