Horse IgG F(ab)2 Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Horse IgG F(ab)2 Antibody Alkaline Phosphatase Conjugated, ALP, Rabbit, Polyclonal
• Artikelnummer: ROC-608-4504
• Hersteller Artikelnummer: 608-4504
• Alternativnummer: ROC-608-4504
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA
• Spezies Reaktivität: Equine
• Immunogen: Horse IgG F(ab)2 fragment
• Konjugation: ALP
• Alternative Synonym: Rabbit Anti-Horse IgG Fab2 Fragment alkaline phosphatase Conjugated Antibody, Rabbit Anti-Horse IgG F(ab)2 Antibody alkaline phosphatase conjugate, Rabbit Anti-Horse IgG Fab2 Antibody alk phos Conjugation
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Horse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:2,000 - 1:8,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,000
• Anwendungsbeschreibung: Anti-Horse IgG F(ab)2 Alkaline Phosphatase conjugate has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring l

Bilder

ELISA Results of Rabbit Anti-Horse IgG F(ab)2 Antibody Alkaline Phosphatase Conjugated tested against purified Horse IgG F(ab)2 Alk Phos. Each well was coated in duplicate with 1.0 µg of Horse IgG F(ab)2 (p/n 008-0102). The working dilution is 14,700. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Alkaline Phosphatase ELISA Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).