Goat IgG anti-Human IgG (H+L)-Biotin, MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Human IgG (H+L)-Biotin, MinX none
• Artikelnummer: ROC-609-1602
• Hersteller Artikelnummer: 609-1602
• Alternativnummer: ROC-609-1602
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG whole molecule
• Konjugation: Biotin
• Alternative Synonym: Goat Anti Human IgG Biotin Conjugated Antibody, Goat Anti-Human IgG Antibody Biotin conjugation
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Human IgG (H&L) Biotin Conjugate has been tested by ELISA and is assayed against 1.0 ug of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code

Bilder

ELISA results of purified Goat Anti-Human IgG Biotin Conjugated Antibody tested against purified human IgG. Each well was coated in duplicate with 1.0 µg of human IgG (purple line). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer MB-060-1000, Streptavidin HRP conjugate 1:10,000, and TMB-8000 substrate.