Goat IgG anti-Mouse IgG2a (Fc)-HRPO, MinX none

Produktübersicht

• Artikelname: Goat IgG anti-Mouse IgG2a (Fc)-HRPO, MinX none
• Artikelnummer: ROC-610-103-041
• Hersteller Artikelnummer: 610-103-041
• Alternativnummer: ROC-610-103-041
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG2a heavy chain
• Konjugation: HRPO
• Alternative Synonym: goat anti-MOUSE IgG2a Antibody peroxidase Conjugation, goat anti-Mouse IgG2a (gamma 2a) HRP Conjugated Antibody
• Format: IgG
• Spezifität: IgG2a (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Anti-Mouse IgG2a Peroxidase antibody has been tested by ELISA and dot blot and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2a antibody based assays. Mouse IgG2a secondary antibody is available in a variety of fo

Bilder

ELISA results of purified Goat anti-Mouse IgG2a Antibody Peroxidase conjugated tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 1.0 µg of Mouse IgG2a [Blue Line]. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate p/n TMBE-1000.