Mouse secondary antibody conjugated to peroxidase is available in a variety of formats. Anti-Mouse IgG Peroxidase Antibody has been tested by ELISA and is suitable for ELISA, Immunohistochemistry western blotting as well as other Horseradish Peroxidase H
ELISA results of purified Goat anti-Mouse IgG Antibody Peroxidase Conjugated (Min x Human Serum Proteins) tested against purified Mouse IgG. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 009-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin and TME ELISA Peroxidase Substrate (p/n TMBE-1000).
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