Goat IgG anti-Mouse IgG3 (Fc)-ATTO 425, MinX none, ATTO-425, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Mouse IgG3 (Fc)-ATTO 425, MinX none, ATTO-425, Polyclonal
• Artikelnummer: ROC-610-151-043
• Hersteller Artikelnummer: 610-151-043
• Alternativnummer: ROC-610-151-043
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG3 heavy chain
• Konjugation: ATTO 425
• Alternative Synonym: goat anti-MOUSE IgG3 Antibody ATTO425 Conjugation, goat anti-MOUSE IgG3 ATTO 425 Conjugated Antibody
• Format: IgG
• Spezifität: IgG3 (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: >1:5,000, WB: 1:4,000 - 1:20,000
• Anwendungsbeschreibung: Mouse IgG3 secondary antibody is available in a variety of formats. Anti-Mouse IgG3 ATTO 425 Antibody has been tested by dot blot. ATTO 425 conjugations are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assay

Bilder

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

ATTO 425 conjugated anti-Mouse IgG was used to demonstrate 2 color STED immunofluorescence microscopy. Methanol fixed A431 cells were blocked with normal goat serum. The cells were then probed with 0.4 µg/mL final concentration of anti-a-tubulin and detected with 0.2 µg/mL ATTO 425 conjugated anti-MOUSE IgG [GOAT] (610-151-121) secondary antibody (colored RED). Also shown in this 2-color STED image is Rocklands Anti-HDAC-1 [RABBIT] (p/n 600-401-879) detected with DyLight(TM)488 conjugated Anti-RABBIT IgG [GOAT] secondary antibody (colored GREEN). Image courtesy of Myriam Gastard, Leica Microsystems, USA.