Goat IgG anti-Mouse IgG2b (Fc)-ATTO 488, MinX none, ATTO-488, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Mouse IgG2b (Fc)-ATTO 488, MinX none, ATTO-488, Polyclonal
• Artikelnummer: ROC-610-152-042
• Hersteller Artikelnummer: 610-152-042
• Alternativnummer: ROC-610-152-042
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG2b heavy chain
• Konjugation: ATTO 488
• Alternative Synonym: goat anti-MOUSE IgG2b Antibody ATTO488 Conjugation, goat anti-Mouse IgG2b (gamma 2b) ATTO 488 Conjugated Antibody
• Format: IgG
• Spezifität: IgG2b (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: >1:5,000, WB: 1:4,000 - 1:20,000
• Anwendungsbeschreibung: Anti-Mouse IgG2b ATTO 488 Antibody has been tested by dot blot. Mouse IgG2b secondary antibody is available in a variety of formats. ATTO 488 Conjugated antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based

Bilder

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Dot Blot of Goat anti-Mouse IgG2b (Gamma 2b Chain) Antibody ATTO 488 Conjugated. Antigen: Mouse IgG2b. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Primary antibody: none. Secondary antibody: Goat anti-Mouse IgG2b (Gamma 2b Chain) Antibody ATTO 488 Conjugated at 1:1,000 for 60 min at RT. Block: MB-070 for 60 min at RT.