Goat IgG anti-Mouse IgG (H+L)-ATTO 488, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, ATTO-488, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Mouse IgG (H+L)-ATTO 488, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Rb,Rt,Sh, ATTO-488, Polyclonal
• Artikelnummer: ROC-610-152-121S
• Hersteller Artikelnummer: 610-152-121S
• Alternativnummer: ROC-610-152-121S
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: FLISA,IF,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG whole molecule
• Konjugation: ATTO 488
• Alternative Synonym: Goat Anti-Mouse IgG Antibody ATTO488 Conjugation, Goat Anti-Mouse IgG ATTO 488 Conjugated Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Rabbit,Rat,Sheep

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Anwendungsbeschreibung: Anti-Mouse IgG ATTO 488 Antibody has been tested by dot blot and western blot. Anti-Mouse IgG (H&L) conjugated by ATTO 488 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent weste

Bilder

Western Blot of ATTO 488 conjugated Goat anti-Mouse IgG Pre-adsorbed secondary antibody. Lane 1: Mouse IgG. Lane 2: none. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: ATTO 488 goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): none.

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH