Mouse IgG2a Antibody ATTO 532 Conjugated Pre-adsorbed, ATTO-532, Goat, Polyclonal

Produktübersicht

• Artikelname: Mouse IgG2a Antibody ATTO 532 Conjugated Pre-adsorbed, ATTO-532, Goat, Polyclonal
• Artikelnummer: ROC-610-153-041
• Hersteller Artikelnummer: 610-153-041
• Alternativnummer: ROC-610-153-041
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: DOT
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG2a heavy chain
• Konjugation: ATTO-532
• Alternative Synonym: Goat anti-mouse IgG2a antibody ATTO532 conjugation, goat anti-mouse IgG2a (gamma 2a) ATTO 532 conjugated antibody
• Format: IgG
• Spezifität: IgG2a (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, Flow Cytometry: 1:500 - 1:2,500, IHC: >1:5,000, IF Microscopy: >1:5,000, WB: >1:10,000
• Anwendungsbeschreibung: Anti-Mouse IgG2a ATTO 532 Antibody has been tested by dot blot. Mouse IgG2a secondary antibody is available in a variety of formats. ATTO dye conjugated antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based

Bilder

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH