Goat IgG anti-Mouse IgG3 (Fc)-ATTO 655, MinX none, ATTO-655, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Mouse IgG3 (Fc)-ATTO 655, MinX none, ATTO-655, Polyclonal
• Artikelnummer: ROC-610-157-043
• Hersteller Artikelnummer: 610-157-043
• Alternativnummer: ROC-610-157-043
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG3 heavy chain
• Konjugation: ATTO 655
• Alternative Synonym: goat anti-MOUSE IgG3 Antibody ATTO655 Conjugation, goat anti-MOUSE IgG3 ATTO 655 Conjugated Antibody
• Format: IgG
• Spezifität: IgG3 (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: >1:20,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: >1:5,000, WB: 1:4,000 - 1:20,000
• Anwendungsbeschreibung: Anti-Mouse IgG3 ATTO 655 Antibody has been tested by dot blot and is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG3 antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wa

Bilder

ATTO dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are: A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody. B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red). C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue). D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain. Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH