Mouse IgG2a Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Mouse IgG2a Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-610-4141
• Hersteller Artikelnummer: 610-4141
• Alternativnummer: ROC-610-4141
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: DOT, ELISA, WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG2a heavy chain
• Konjugation: Unconjugated
• Alternative Synonym: Rabbit Anti-Mouse IgG2a Antibody, Mouse IgG2a Secondary Antibody, Rb-a-mouse IgG2a
• Format: IgG
• Spezifität: IgG2a (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.02 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:15,000 - 1:30,000, IHC: 1:2,000 - 1:10,000, WB: 1:4,000 - 1:20,000
• Anwendungsbeschreibung: Anti-MOUSE IgG2a (Gamma 2a chain) (RABBIT) Antibody has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Bilder

Western Blot of Rabbit anti-Mouse IgG2a antibody. Lane 1: Mouse IgG1 (p/n 010-0102). Lane 2: Mouse IgG2a (p/n 010-0141). Lane 3: Mouse IgG2b (p/n 010-0142). Lane 4: Mouse IgG3 (p/n 010-0143). Lane 5: Mouse IgM (p/n 010-0107). Load: 5 ng per lane. Primary antibody: Mouse IgG2a antibody at 1 µg/mL for overnight at 4C. Secondary antibody: Gt-anti-Rabbit HRP (p/n 611-103-122) at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.

Dot Blot of Rabbit Anti-Mouse IgG2a Antibody. Tested with dilutions 10ng, 3.3ng, 1.1ng, 0.37ng, 0.12ng. Lane 1: Mouse IgG1 (p/n 010-0102). Lane 2: Mouse IgG2a (p/n 010-0141). Lane 3: Mouse IgGb (p/n 010-0142). Lane 4: Mouse IgG3 (p/n 010-0143). Lane 5: Mouse IgM (p/n 010-0107). Primary Antibody: Rabbit anti-Mouse IgG2a at 1µg/mL for 1 hour at RT. Secondary Antibody: Gt-anti-Rabbit HRP (p/n 611-103-122) at 1:40,000. Blocking Buffer: BlockOut MB-073 for 30 min at RT.