Rabbit IgG anti-Mouse IgG (H)-Alk. Phos., MinX none, ALP, Polyclonal , AP

Produktübersicht

• Artikelname: Rabbit IgG anti-Mouse IgG (H)-Alk. Phos., MinX none, ALP, Polyclonal , AP
• Artikelnummer: ROC-610-4512
• Hersteller Artikelnummer: 610-4512
• Alternativnummer: ROC-610-4512
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Mouse
• Immunogen: Mouse IgG gamma heavy chain
• Konjugation: Alk. Phos.
• Alternative Synonym: rabbit anti-Mouse IgG Antibody alkaline phosphatase conjugation, rabbit anti-Mouse IgG gamma alk phos conjugated Antibody
• Format: IgG
• Spezifität: IgG (Fc)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol, pH 8.0
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Mouse
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:1,000 - 1:11,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Anwendungsbeschreibung: Anti-Mouse IgG (gamma chain) Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivit

Bilder

ELISA Results of Rabbit Anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated tested against purified Mouse IgG Alk Phos. Each well was coated in duplicate with 1.0 µg of Mouse IgG (p/n 010-0102). The working dilution is 1:9000. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using ELISA Alkaline Phosphatase Substrate Buffer (p/n NPP-10) and NPP Working Buffer (p/n NPP-B500).