Goat IgG anti-Rabbit IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Rabbit IgG (H+L)-RPE, MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal
• Artikelnummer: ROC-611-108-122
• Hersteller Artikelnummer: 611-108-122
• Alternativnummer: ROC-611-108-122
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: FACS,IF
• Spezies Reaktivität: Rabbit
• Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG whole molecule in goat.
• Konjugation: RPE
• Alternative Synonym: goat anti-Rabbit IgG Antibody, Gt-a-Rabbit Phycoerythrin conjugated, Rabbit IgG (H&L) Antibody in goat, Goat IgG (H&L) Phycoerythrin conjugated Secondary Antibody.
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 0.5 mg/mL by absorbance = 82.0 at 565 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: Flow Cytometry: 1:100 - 1:250, IF Microscopy: 1:100 - 1:250
• Anwendungsbeschreibung: Anti-Rabbit IgG (H&L) Phycoerythrin Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of

Bilder

Western Blot of Goat anti-Rabbit IgG Pre-Absorbed Phycoerythrin Conjugated Antibody. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Phycoerythrin goat secondary antibody at 1:1,000 for 60 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 28 & 55 kDa, 55 kDa for Rabbit IgG. Other band(s): None.

Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.