Goat IgG anti-Rabbit IgG (H+L)-HRPO, MinX Hu, Polyclonal

Produktübersicht

• Artikelname: Goat IgG anti-Rabbit IgG (H+L)-HRPO, MinX Hu, Polyclonal
• Artikelnummer: ROC-611-1322
• Hersteller Artikelnummer: 611-1322
• Alternativnummer: ROC-611-1322
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Konjugation: HRPO
• Alternative Synonym: goat anti-Rabbit IgG Antibody peroxidase conjugated, Gt-a-Rabbit peroxidase conjugated IgG, Rabbit peroxidase conjugated Antibody in Goat, Rabbit Secondary peroxidase conjugated Antibody, HRP secondary, peroxidase secondary
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Human

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:50,000 - 1:100,000, IHC: 1:750 - 1:2,500, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Goat Anti-Rabbit IgG peroxidase conjugated antibody has been tested by ELISA and western blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antib

Bilder

Western Blot of Goat anti-Rabbit IgG Peroxidase Conjugated Antibody. Lane 1: HeLa Whole Cell Lysate. Lane 2: NIH 3T3 Whole Cell Lysate. Load: 10 µg per lane. Primary antibody: Beta Actin antibody at 1:2,000 for overnight at 4C. Secondary antibody (2 Blots on Left): Atto 647N goat secondary antibody at 1:10,000 for 60 min at RT. Secondary Antibody (2 Blots on Right): Peroxidase goat secondary antibody at 1:10,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 50 kDa, 50 kDa for Beta Actin. Other band(s): none.

Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.