Rabbit IgG F(ab)2 Antibody Peroxidase Conjugated Pre-Adsorbed, HRP, Goat, Polyclonal

Produktübersicht

• Artikelname: Rabbit IgG F(ab)2 Antibody Peroxidase Conjugated Pre-Adsorbed, HRP, Goat, Polyclonal
• Artikelnummer: ROC-611-1324
• Hersteller Artikelnummer: 611-1324
• Alternativnummer: ROC-611-1324
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA
• Spezies Reaktivität: Rabbit
• Immunogen: Anti-Rabbit IgG F(ab)2 fragment was produced by repeated immunization with Rabbit IgG F(ab)2 fragment in goat.
• Konjugation: HRP
• Alternative Synonym: Goat Anti-Rabbit IgG F(ab)2 Antibody peroxidase Conjugation, Goat Anti-Rabbit IgG Fab2 peroxidase Conjugated Antibody, Goat Anti-Rabbit IgG Fab2 Fragment HRP Conjugated Antibody
• Format: IgG
• Spezifität: IgG (F(ab')2)
• Minimale Kreuzreaktivität (MinX): Human

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:130,000, IHC: 1:1,000 - 1:10,000, WB: 1:2,000 - 1:20,000
• Anwendungsbeschreibung: Anti-Rabbit IgG F(ab)2 fragment has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optim

Bilder

Western Blot of Anti-Rabbit IgG F(ab)2 (GOAT) Antibody (p/n 611-1104). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG F(ab)2 (GOAT) Antibody (p/n 611-1104) 1:10,000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody (p/n CUST10) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.