Mouse IgG anti-Rabbit IgG (H+L)-FITC, MinX Hu,Go,Ms, Polyclonal

Produktübersicht

• Artikelname: Mouse IgG anti-Rabbit IgG (H+L)-FITC, MinX Hu,Go,Ms, Polyclonal
• Artikelnummer: ROC-611-302-122
• Hersteller Artikelnummer: 611-302-122
• Alternativnummer: ROC-611-302-122
• Hersteller: Rockland Immunochemicals
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: FLISA,FACS,IF
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Konjugation: FITC
• Alternative Synonym: Mouse Anti-Rabbit IgG Fluorescein Conjugated Antibody, Mouse Anti Rabbit IgG Antibody FITC Conjugation
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Human,Goat,Mouse

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Lyophilized
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: FLISA: 1:10,000 - 1:50,000, Flow Cytometry: 1:500 - 1:2,500, IF Microscopy: 1:1,000 - 1:5,000
• Anwendungsbeschreibung: Mouse Anti-Rabbit IgG (H&L) Antibody Fluorescein Conjugated has been tested by ELISA and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex

Bilder

Western Blot of Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-301-122). Lane M: 3 µl Molecular Ladder. Lane 1: Rabbit IgG whole molecule (p/n 011-0102). Lane 2: Rabbit IgG F(ab) Fragment (p/n 011-0105). Lane 3: Rabbit IgG F(c) Fragment (p/n 010-0103). Lane 4: Rabbit IgM Whole Molecule (p/n 011-0107). Lane 5: Normal Rabbit Serum (p/n B309). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (MOUSE) Antibody (Min X Hu, Gt, Ms Serum Proteins) (p/n 611-301-122) 1:1,000 for 60 min at RT. Secondary antibody: Anti-Mouse IgG (RABBIT) Peroxidase Conjugated Antibody (p/n 610-403-C46) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.