Anti-Rat IgA Biotin Conjugated Antibody has been tested by western blot and assayed against 1.0 ug of Rat IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin S000-03 and ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) c
Western Blot of Biotin Conjugated Goat Anti-Rat IgA (Alpha chain) Secondary Antibody. Lane 1: Molecular Weight Marker. Lane 2: Mouse IgA Kappa. Load: 100 ng per lane. Primary antibody: Rat IgA (Alpha chain) antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): None.
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