Rat IgG (H&L) Antibody Pre-adsorbed, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Rat IgG (H&L) Antibody Pre-adsorbed, Rabbit, Polyclonal
• Artikelnummer: ROC-612-4126
• Hersteller Artikelnummer: 612-4126
• Alternativnummer: ROC-612-4126
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, WB
• Spezies Reaktivität: Rat
• Immunogen: Anti-Rat IgG (H&L) was produced by repeated immunization with rat IgG whole molecule in rabbit
• Konjugation: Unconjugated
• Alternative Synonym: rabbit anti-Rat IgG (H&L) Antibody, Rabbit-a-Rat IgG (H&L), Rat IgG Antibody in rabbit, anti-Rat IgG Secondary Antibody
• Format: IgG
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Human

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 2.2 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Rat
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000 - 1:300,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
• Anwendungsbeschreibung: Anti-Rat IgG has been tested by ELISA and western blot and has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 minutes at room tem

Bilder

Western Blot of Anti-Rat IgG (H&L) (RABBIT) Antibody (Min X Human Serum Proteins) (p/n 612-4126). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n 012-0102). Lane 2: Rat IgG F(c) Fragment (p/n 012-0103). Lane 3: Rat IgG Fab Fragment (p/n 012-0105). Lane 4: Rat IgM Whole Molecule (p/n 012-0107). Lane 5: Rat Serum (p/n D310-05). All samples were reduced. Load: 50 ng per lane. Block: MB-070 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (RABBIT) Antibody (Min X Human Serum Proteins) (p/n 612-4126) 1:1,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n 611-103-122) 1:40,000 in MB-070 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.