F(ab)2 Anti-Guinea Pig IgG Peroxidase Antibody has been tested by ELISA and is assayed against 1.0 ug of Guinea Pig IgG in a standard capture ELISA using ABTS (2,2-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code ABTS-100 as a substrate for 30 m
ELISA Results of F(ab)2 Goat Anti-Guinea Pig IgG Antibody Peroxidase Conjugated. Each well was coated in duplicate with 1.0 µg of Guinea Pig IgG (p/n 006-0102). The working dilution is 93,100. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS (p/n MB-066) and TMB substrate (p/n TMBE-1000).
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