Goat F(ab)2 anti-Rabbit IgG (H+L)-HRPO, MinX none

Artikelnummer: ROC-711-103-002
Artikelname: Goat F(ab)2 anti-Rabbit IgG (H+L)-HRPO, MinX none
Artikelnummer: ROC-711-103-002
Hersteller Artikelnummer: 711-103-002
Alternativnummer: ROC-711-103-002
Hersteller: Rockland Immunochemicals
Wirt: Goat
Kategorie: Antikörper
Applikation: ELISA,IHC,WB
Spezies Reaktivität: Rabbit
Immunogen: Rabbit IgG whole molecule
Konjugation: HRPO
Alternative Synonym: Goat F(ab)2 Anti-Rabbit IgG Peroxidase Conjugated Antibody, Goat Fab2 Anti-Rabbit IgG Antibody HRP Conjugation
Format: F(ab')2
Spezifität: IgG (H+L)
Minimale Kreuzreaktivität (MinX): no cross-adsorbtion
Klonalität: Polyclonal
Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Formulierung: Lyophilized
Target-Kategorie: Rabbit
Antibody Type: Secondary Antibody
Application Verdünnung: ELISA: 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:2,000 - 1:10,000
Anwendungsbeschreibung: F(ab)2 Anti-Rabbit IgG Peroxidase Conjugated Antibody has been tested by ELISA and is ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
ELISA Results of Goat F(ab)2 Anti-Rabbit IgG Antibody Peroxidase Conjugated. Each well was coated in duplicate with 10 µg of Rabbit IgG (p/n 011-0102). The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/ PBS (p/n MB-066) and TMB substrate p/n TMBE-1000.