Goat F(ab)2 anti-Rabbit IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal , Unconjugated

Produktübersicht

• Artikelname: Goat F(ab)2 anti-Rabbit IgG (H+L)-unconj., MinX Bo,Ck,Go,Gp,Hm,Ho,Hu,Ms,Rt,Sh, Polyclonal , Unconjugated
• Artikelnummer: ROC-711-1122
• Hersteller Artikelnummer: 711-1122
• Alternativnummer: ROC-711-1122
• Hersteller: Rockland Immunochemicals
• Wirt: Goat
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Rabbit
• Immunogen: Rabbit IgG whole molecule
• Konjugation: Unconjugated
• Alternative Synonym: Goat F(ab)2 Anti-Rabbit IgG Antibody Pre-Adsorbed, Goat Fab2 Anti-Rabbit IgG Antibody
• Format: F(ab')2
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): Bovine,Gallus,Goat,Guinea pig,Hamster (all),Equine,Human,Mouse,Rat,Sheep

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Rabbit
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:20,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: F(ab)2 Anti-Rabbit IgG Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, h

Bilder

Western Blot results using Goat F(ab)2 Anti-Rabbit IgG.Effects of RAB38 silencing on the proliferation ability of PANC-1 and BxPC-3 cells. The expression levels of the proliferation-associated proteins (C) Ki67 and (D) PCNA in PANC-1 and BxPC-3 cells were detected by western blot analysis. Data are reported as the mean standard deviation (n=3). *P<0.05 vs. control group. shRNA, small hairpin RNA, RAB38, Ras-related GTP-binding protein 38, OD, optical density, PCNA, proliferating cell nuclear antigen. Figure 3. PMID: 30569114.

SDS-PAGE results of Goat F(ab)2 Anti-Rabbit IgG (H&L) Antibody. Lane 1: reduced F(ab)2 anti-Mouse (lot 36997). Lane 2: reduced F(ab)2 anti-Rabbit (lot 36998). Lane 3: Opal Prestained Ladder - MB-210-0500. Lane 4: non-reduced F(ab)2 anti-Mouse. Lane 5: non-reduced F(ab)2 anti-Rabbit. Load: 1.0ug. 4-20% Lonza SDS-PAGE, Coomassie Stained, BioRad ChemiDoc Imaged.

KIF20B shRNA efficiency. (B) The shRNA efficiency measured by western blotting. Primary antibodies used rabbit anti-KIF20B antibody [1:100] and mouse anti-beta-actin [1:500] with goat anti-rabbit [1:10,000] p/n 711-1122 and goat anti-mouse Fc secondary antibody [1:10,000] p/n 110-1103. *P<0.05 vs. control. KIF, kinesin family member, shRNA, short hairpin RNA. Figure 2. PMID: 30664160.