F(ab)2 Anti-Rat IgG F(c) Fluorescein Antibody has been tested by dot blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(
Dot Blot results of Goat F(ab)2 Anti-Rat IgG Antibody Fluorescein Conjugated. Dots are Rat F(c) at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Blocking: MB-070 for 60 min at RT. Primary Antibody: none. Secondary Antibody: Goat F(ab)2 Anti-Rat IgG Antibody FITC at 1µg/mL for 1hr at RT. Imaged with BioRad ChemiDoc, FITC filter.
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