Fab Anti-Goat IgG Fluorescein Antibody has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c)
Western blot of Fluorescein conjugated Donkey Fab Anti-Goat IgG secondary antibody. Lane 1: Molecular Weight Marker. Lane 2: Goat IgG. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein donkey secondary antibody at 1:1,000 for 60 min at RT. Blocking: MB-070 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG. Other band(s): None.
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