Fab Human IgG (H&L) Antibody, Rabbit, Polyclonal

Produktübersicht

• Artikelname: Fab Human IgG (H&L) Antibody, Rabbit, Polyclonal
• Artikelnummer: ROC-809-4102
• Hersteller Artikelnummer: 809-4102
• Alternativnummer: ROC-809-4102
• Hersteller: Rockland Immunochemicals
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA,IHC,WB
• Spezies Reaktivität: Human
• Immunogen: Human IgG whole molecule
• Konjugation: Unconjugated
• Alternative Synonym: Rabbit Fab Anti-Human IgG Antibody, Rabbit Fab Fragment Anti-Human IgG Antibody
• Format: Fab
• Spezifität: IgG (H+L)
• Minimale Kreuzreaktivität (MinX): no cross-adsorbtion

Produkteigenschaften

• Klonalität: Polyclonal
• Konzentration: 1.0 mg/mL by UV absorbance at 280 nm
• Puffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Formulierung: Liquid (sterile filtered)
• Target-Kategorie: Human
• Antibody Type: Secondary Antibody

Anwendungsbeschreibung

• Application Verdünnung: ELISA: 1:30,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Anwendungsbeschreibung: Fab Anti-Human IgG (H&L) Antibody is suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Bilder

Fluorescence microscopy using Rabbit Fab Anti-Human IgG. Analysis of human IgGs displayed on the surface of OCMS(TM) hybridomas. (a) 8C5 OCMS(TM) (top row) and B5-6T OCMS(TM) (bottom row) cells were incubated overnight with the RAH (AB14), followed by biotinylated rabies GP and Alexa Fluor 488 streptavidin (green) (AB3), and visualized by fluorescence microscopy. Images are shown with DAPI (blue) (right) or without DAPI (left). Scale bar=5µm. (b) The 4G4 OCMS(TM) (top row) and 8C5 OCMS(TM) (bottom row) hybridomas were incubated overnight with a rabbit Fab anti-human IgG (AB17), then for an hour with additional rabbit Fab anti-human IgG and Sabin type III PV (left column) or GP (right column), followed by an Alexa Fluor 488 anti-human IgG (green) (AB2). Nuclei were stained with DAPI (blue). Scale bar=10µm. (c) In this mixing experiment, non-secretor OCMS(TM) cell lines were labeled with CFSE (green) and mixed in a 10:1 ratio with IgG-secreting OCMS(TM) hybridomas. Cells were incubated overnight with rabbit F(ab)2anti-human IgG (AB15) then washed and incubated with biotinylated Protein A (AB12) and APC streptavidin (red) (AB4). (Top row) 8C5 OCMS(TM) vs. B5-6T OCMS(TM) CFSE. (Bottom row) 4G4 OCMS(TM) vs. LCX OCMS(TM) CFSE. With DAPI (blue) (right column) or without (left column). Scale bar=5µm. Figure 2. PMID: 30794061.