Recombinant protein corresponding to Phe337-Arg654 of mouse SULF2, expressed in E. coli.
Sulfatase 2contains an N-terminal signal sequence, followed by a sulfatase domain, a hydrophilic region, and a C-terminal substrate recognition domain.SULF2 shares about 64% identity with SULF1 and 94% identity with mouse Sulf2. PCR detected SULF2 expression in most human tissues examined, with highest levels in ovary, skeletal muscle, stomach, brain, uterus, heart, kidney, and placenta. Western blot analysis of transfected Chinese hamster ovary (CHO) cells and culture medium detected SULF2 proteins with apparent molecular masses of 132, 64, and 60kD in the culture medium. Treatment with furin inhibitors resulted in a single 132kD species, and treatment with N-glycanase reduced the molecular mass from 132kD to 100kD. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
