Apaf1 (Apoptosis Protease Activating Factor 1, Apaf-1), Clone: [6G50], Mouse, Monoclonal
Artikelnummer:
USB-A2298-64L
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| Artikelname: | Apaf1 (Apoptosis Protease Activating Factor 1, Apaf-1), Clone: [6G50], Mouse, Monoclonal |
| Artikelnummer: | USB-A2298-64L |
| Hersteller Artikelnummer: | A2298-64L |
| Alternativnummer: | USB-A2298-64L-100 |
| Hersteller: | US Biological |
| Wirt: | Mouse |
| Kategorie: | Antikörper |
| Applikation: | IHC, WB |
| Immunogen: | Synthetic peptide corresponding to an aa-sequence near the C-terminal of human Apaf1. |
| Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease- activating factor 1 (1,2). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 transcript is ubiquitously expressed in human tissues (1). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.25-0.5ug/ml. Detect a band at ~130kD in K562 cell lysate. Immunohistochemistry: 0.5ug/ml. Optimal dilutions to be determined by the researcher. Positive Control: K562 cell lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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