Vitronectin receptor protein from the mouse T-cell hybridoma 2B4
CD61 is a 110kD integrin b chain also known as b3 integrin or gpIIIa. It associates with the integrin av chain (CD51) to form the vitronectin receptor. In addition, CD61 can associate with the integrin aIIb chain (CD41) to form the gpIIb/IIIa complex. CD61 is expressed on platelets, megakaryocytes, endothelium, smooth muscle, a subset of B cells, myeloid cells, osteoclasts, and mast cells. CD61, in conjunction with CD41 or CD51, mediates adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. Leukocyte-endothelial adhesion is mediated by the binding of av/b3 integrin or vitronectin receptor to CD31 (PECAM-1). This antibody reacts with mouse and rat CD61, and is able to block ligand binding. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e6 cells in 100ul volume Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
