Synthetic peptide corresponding to C-KNIHLEKKYVR, from human CSF1R, at the internal region of the protein (NP_005202.2).
Colony-stimulating factor 1(CSF-1) is required for the growth, differentiation and survival of cells of the monocyte/macrophage lineage. CSF-1 binds specific receptor CSF1R, the product of the c-fms gene, leading to receptor dimerization and phosphorylation on tyrosine residue. CSF-1R has been demonstrated as the major regulator of macrophage development as well as great involvement in epithelial cancers, including carcinogenesis of breast, ovary and prostate. CSF- 1R is a tyrosine kinase transmembrane receptor and is one of the members of the CSF1/PDGF receptor family of tyrosine-protein kinases. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:32,000 Western Blot: 0.1-0.5ug/ml, transfected HEK293 transiently expressing full-length Human CSF1R (myc and DYKDDDDK tagged) observed on ~200kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
