Flt3 Ligand, Recombinant, Human (Fms-related Tyrosine Kinase 3 Ligand, Flt3L)
Artikelnummer:
USB-F4600-11
Hersteller Artikelnummer:
F4600-11
Alternativnummer:
USB-F4600-11-2,USB-F4600-11-10
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Flt3-Ligand is a growth factor that regulates proliferation of early hematopoietic cells. Flt3-Ligand binds to cells expressing the tyrosine kinase receptor Flt3. Flt3-Ligand, by itself does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation. Unlike SCF, Flt3-Ligand exerts no activity on mast cells. Multiple isoforms of Flt3-Ligand have been identified. The predominant biologically active form is anchored to the cell surface as the extracellular domain of a transmembrane protein (209 a.a.). The membrane-bound isoform can be proteolytically cleaved to generate a biologically active soluble isoform. Recombinant human Flt3-Ligand is a soluble 17.6kD protein consisting of 155 amino acid residues. Amino Acid Sequence: TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTA Biological Activity: The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is 1ng/ml, corresponding to a specific activity of 1x10e6 units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplied as a lyophilized powder without additives. Reconstitute with sterile ddH2O, 0.1% HSA or BSA >0.1mg/ml, which can then be further diluted to other aqueous solutions.
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