Synthetic peptide corresponding to aa1-50 of human p65 protein. Species Sequence Homology: canine, mouse and rat
NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65. Applications: Suitable for use in Immunofluorescence, Immunocytochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:100-1:2000 Immunofluorescence (IC): 10ug/ml Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.