Plasmin Substrate (Mes-D-LGR-ANSN(C2H5)2)

Produktübersicht

• Artikelname: Plasmin Substrate (Mes-D-LGR-ANSN(C2H5)2)
• Artikelnummer: USB-P4256-11
• Hersteller Artikelnummer: P4256-11
• Alternativnummer: USB-P4256-11-1
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Substrates containing the fluorescent reporter group 6-amino-1-naphthalene-sulfonamide (ANSN) are useful compounds for monitoring the enzyme activity of various serine proteases (1-12). In this class of compounds, the ANSN reporter group is linked (in the R1 position) to short tri-peptide sequences. The peptide sequences are designed to optimize the interaction between the enzyme and substrate. Additional components which may be added to the R2 and R3 positions reflect changes in the P subsite positions, and generally affect the kinetic parameters of the substrates. Compounds which are effective substrates are hydrolyzed between the tri-peptide and the ANSN group. Once cleaved from the peptide moiety, the ANSN group exhibits about a 1000 fold increase in relative fluorescence. The kinetic properties listed below will aid in the selection of an appropriate substrate. The ANSN substrates have proved especially useful for the analyses of factor VIIa (1-5,7,9,10,12). Although the substrate hydrolysis rates are relatively slow for factor VIIa alone, several compounds exhibit a large change in kcat when tissue factor is incorporated into the assay system. Form: Supplied as a liquid in DMSO. Concentration: 7.8mg/ml Assays physiologic buffers: Hepes or Tris, with substrate concentrations ranging from 1-100uM Emission Wavelength: 470nm Excitation Wavelength: 352nm Light artifacts: Minimized by using a390-450nm long-pass cutoff filter in the emission beam. Kinetic Properties of Related Substrates: (KM - uM, kcat - s-1, kcat/KM - M-1s-1x10e-5) IIa VIIa VIIa/TF Xa XIa aPC tPA Plasmin Mes-D-LGR-ANSN(C2H5)2 KM ND ND ND ND ND ND ND 130 F.W. = 682.8 kcat ND ND ND ND ND ND ND 3.7 (useful for plasmin) kcat/KM ND ND ND ND ND ND ND 0.28 Mes-D-LGR-ANSN(C2H5)2 KM 31 180 200 125 580 113 ND ND F.W. = 682.8 kcat 0.63 0.007 0.79 36 15 0.055 ND ND (useful for factor Xa) kcat/KM 0.2 0.0004 0.04 2.9 0.26 0.005 ND ND D-LPR-ANSNH-C3H7 2 HCl KM 0.5 300 300 171 75 45 98 ND F.W. = 721.74 kcat 19 0.07 4.5 3.3 53 52 0.31 ND (useful for factor XIa) kcat/KM 380 0.002 0.15 0.19 7.1 12 0.03 ND D-FPR-ANSNH-C6H112HCl KM 0.4 150 330 150 ND 7.8 36 ND F.W. = 777.81 kcat 17 0.05 8.4 0.32 ND 6.6 0.074 ND (useful for IIa & factor VIIa) kcat/KM 430 0.004 0.25 0.02 ND 8.6 0.02 ND D-FPR-ANSNH-C4H9 2 HCl KM ND 186 102 ND ND 53 ND ND F.W. = 751.76 kcat ND 0.11 2.7 ND ND 21 ND ND (useful for factor VIIa) kcat/KM ND 0.006 0.26 ND ND 4 ND ND Boc-L-(p-F)FPR-ANSNH-C2H5 KM 3.7 50 217 ND ND ND 71 ND F.W. = 768.92 kcat 44 0.008 0.88 ND ND ND 1.03 ND (useful for tPA) kcat/KM 120 0.002 0.04 ND ND ND 0.15 ND Boc-L-FPR-ANSNH-C2H5 KM 17 ND ND 100 ND 540 47 ND F.W. = 750.90 kcat 58 ND ND 0.31 ND 2.2 0.011 ND (useful for thrombin) kcat/KM 34 ND ND 0.003 ND 0.04 0.002 ND L-EGR-ANSNH-C3H7 2 HBr KM 100 ND ND 110 225 440 ND ND F.W. = 724.6 kcat 2.5 ND ND 0.2 82 17 ND ND (useful for factor XIa) kcat/KM 0.25 ND ND 0.02 3.6 0.39 ND ND BOC-D-VLR-ANSNH-C4H9 KM 19 42 170 19 ND 3.9 ND ND F.W. = 746.98 kcat 0.055 0.007 1.6 0.055 ND 2.1 ND ND (useful for aPC) kcat/KM 0.03 0.002 0.09 0.03 ND 5.2 ND ND D-VPR-ANSNH-C4H9 2 HCl KM 2 89 52 160 520 54 110 ND F.W. = 703.73 kcat 110 0.019 0.76 3.3 92 72 0.71 ND (useful for thrombin and aPC) kcat/KM 550 0.002 0.14 0.21 1.8 13 0.065 ND ND = NOT DETERMINED Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.