2E10 was generated by immunizing BALB/c mice with a DNA vaccine containing the human alpha2 I-domain cDNAsequence within pVAC2. The alpha2 I-domain is expressed as a fusion protein with fragment C of tetanus toxoid (FrC-TT). The scFv was then created using a V-gene phage display library derived from RNA from mice splenocytes and isolated following selection on GST-I after 3 rounds of panning.