Anti-Brg1 [4E5 ], Rat IgG2b, kappa, Clone: [4E5], Monoclonal
Artikelnummer:
ABA-AB00905-8.1
Hersteller Artikelnummer:
Ab00905-8.1
Alternativnummer:
ABA-AB00905-8.1
Hersteller:
Absolute Antibody
Wirt:
Rat
Kategorie:
Antikörper
Applikation:
IF, IHC, IP, WB
Spezies Reaktivität:
Human, Mouse
Alternative Synonym:
SMARCA4, alpha-BRG1, BAF190, BAF190A, BRG1, MRD16, RTPS2, SNF2, SNF2L4, SNF2LB, SWI2, hSNF2b, CSS4, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Anti-Brg1 rat monoclonal antibodies were generated based on the rat lymph node method. An 8-week-old female lzm rat was injected via the hind footpads with 200 mL of emulsion containing 100 mg of recombinant mouse Brg1 protein and Freunds complete adjuvant. After 3 weeks, the cells from the lymph nodes of a rat immunized with an antigen were fused with mouse myeloma Sp2/0-Ag14 cells at a ratio of 5:1 in a 50% polyethylene glycol solution. The resulting hybridoma cells were plated onto 96-wellplates and cultured in HAT selection medium. After 10 days post-fusion, the hybridoma supernatants were screened by means of an ELISA against GST-fused Brg1. Positive clones were subcloned and rescreened by ELISA and immunoblotting.
