The original human IgG1 version of this antibody was obtained in the following manner: First, white matter tissue was dissected at autopsy from subjects with clinically defined MS, and individual B lineage cells were identified and captured. Multiplex PCR was used to amplify the Ig heavy and light chain variable regions, and recombinant IgGs were prepared from the Ig variable region sequences with IgG1 heavy chain and kappa constant domains.
