ASGPR, ASGP-R, ASGR1, C-Type Lectin Domain Family 4 Member H1, Hepatic Lectin H1, CLEC4H1, HL-1,
A human non-immune phage antibody library was screened with the recombinant carbohydrate recognition domain of rCRDH1 (the H1 subunit of human asialoglycoprotein receptor). Anti-rCRDH1 phage clones were obtained after 4 rounds of screening with rCRDH1-coated immunotubes and single positive colonies were further selected with the expressed thioredoxin-His-S tag of the wild-type pET32c. Specific anti-rCRDH1 phage clones were transfected into E. coli HB2151 and induced for secreted expression of scFv antibody.
