Anti-CD131 [BION-1], Human IgG1, Fc Silent(TM), kappa, Monoclonal
Artikelnummer:
ABA-AB00957-10.3
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| Artikelname: | Anti-CD131 [BION-1], Human IgG1, Fc Silent(TM), kappa, Monoclonal |
| Artikelnummer: | ABA-AB00957-10.3 |
| Hersteller Artikelnummer: | Ab00957-10.3 |
| Alternativnummer: | ABA-AB00957-10.3 |
| Hersteller: | Absolute Antibody |
| Wirt: | Human |
| Kategorie: | Antikörper |
| Applikation: | Blocking, Crystallography, Functional Studies, IHC, IP |
| Spezies Reaktivität: | Human |
| Alternative Synonym: | CSF2RB, CD131, IL3RB, IL-3R common beta subunit, IL5RB, colony stimulating factor 2 receptor, beta, low-affinity (granulocyte-macrophage), SMDP5, Common Beta-Chain, CDw131, cytokine receptor common subunit beta, beta-c, CD131, Beta Common Chain |
| BALB/c mice were immunized intraperitonally with COS cells transfected DQP (Domain 4 of the common beta-chain) expression constructs. The immunizations were repeated 4 times at 2-week intervals. Four weeks after the final immunization, a mouse was boosted with COS transfectants intravenously. Three days later, splenocytes were harvested and fused with NS-1 myeloma cells. Hybridoma supernatants were screened on CHO DQP cells by flow cytometry, with untransfected CHO cells as a control. All antibodies were from single hybridoma clones as selected by a limiting dilution method. MoAbs were purified from ascites fluid or hybridoma supernatant by a protein A sepharose column. |
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