G. rostochiensis, Globodera rostochiensis, G. rostochiensis, MRG 48, MGR-48,
Sixteen mice were immunised intraperitoneally with isolated protein fractions from homogenised preparasitic second-stage juveniles of G. rostochiensis, which were mixed 1:1 with Freunds incomplete adjuvant. weeks later antiserum samples were collected for immunofluorescence microscopy. One mouse that was selected for monoclonal antibody production received a final booster injection 12 weeks after the first immunization. Three days later the mouse was sacrificed, and MAb-producing hybridoma cell lines were obtained by fusing spleen cells with SP 2/0 myeloma cells.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[MGR48]
Isotyp:
IgG
Puffer:
PBS only.
Quelle:
Mouse
Target-Kategorie:
Potato cyst nematode
Antibody Type:
Recombinant Antibody
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