gUG1, colony-forming unit Hill cells, CFU-Hills, circulating angiogenic cells, CACs, endothelial colony-forming cells, ECFCs, early outgrowth cells, early EPCs, late outgrowth cells, late EPCs
Peripheral blood was obtained from human donors. The PBMCs were obtained by density centrifugation and cultured. Two ISA brown chickens were then injected intradermally and after 50 days the spleen and bone marrow was harvested. RT-PCR was performed on cells from the spleen and bone marrow which was used to generate a cDNA library. Primers were then used to amplify the VL and VH genes and this was assembled in an scFv format. Phage libraries were generated and the library was biopanned. The biopanned library was then transformed into XL1 blue bacteria and colonies chosed at random from these.
