The antibody was generated by immunizing Sprague Dawley rats and half-lop rabbits with various antigens, including synthetic peptides mapping to regions common to both TcdA and TcdB full-length toxins, formaldehyde-inactivated toxoid A, binding domain fragments of Toxin A, binding domain fragments of Toxin B, or combinations of these. Following immunization, B cells were isolated, and the resulting antibodies were subsequently humanized.
