SYNE3 Rabbit pAb, Unconjugated

Artikelnummer: ABB-A13237
Artikelname: SYNE3 Rabbit pAb, Unconjugated
Artikelnummer: ABB-A13237
Hersteller Artikelnummer: A13237
Alternativnummer: ABB-A13237-20UL,ABB-A13237-100UL,ABB-A13237-500UL,ABB-A13237-1000UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, WB
Spezies Reaktivität: Human
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Konjugation: Unconjugated
Alternative Synonym: KASH3, NET53, Nesp3, C14orf49, C14orf139, LINC00341, NCRNA00341, SYNE3
Enables actin filament binding activity and cytoskeleton-nuclear membrane anchor activity. Involved in cytoskeleton organization, establishment of protein localization to membrane, and regulation of cell shape. Located in nuclear membrane. Part of meiotic nuclear membrane microtubule tethering complex. Biomarker of Huntingtons disease.
Klonalität: Polyclonal
Molekulargewicht: 112kDa
NCBI: 161176
UniProt: Q6ZMZ3
Reinheit: Affinity purification
Sequenz: LRLLEESLLSLIRNWHLQRMEVDSGKKMVFTNNIPKSGFLINPMDPIPRHRRRANLLQEEEGSHEDFSQLLRNFGQWLQVENSKLVRIIAMRTSTAEDLRTRKSKLQELEARVPEGQHLFENLLRLGPARGTSDELEDLRYQWMLYKSKLKDSGHLLTQSSPGEPTGFQKTRRWRGLGSLF
Target-Kategorie: SYNE3
Antibody Type: Primary Antibody
Application Verdünnung: WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse, ResearchArea: Cytoskeleton,Microfilaments.
Immunofluorescence analysis of HeLa cells using SYNE3 Rabbit pAb (A13237) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using SYNE3 Rabbit pAb (A13237) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Western blot analysis of various lysates using SYNE3 Rabbit pAb (A13237) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.