SARS-CoV-2 Spike S1 mAb, Rabbit

Artikelnummer: ABB-A20022
Artikelname: SARS-CoV-2 Spike S1 mAb, Rabbit
Artikelnummer: ABB-A20022
Hersteller Artikelnummer: A20022
Alternativnummer: ABB-A20022-100UL, ABB-A20022-20UL
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: ELISA, IF, WB
Spezies Reaktivität: Virus
Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Alternative Synonym: sars-cov-2, spike glycoprotein
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.
NCBI: 43740568
Reinheit: Affinity purification
Target-Kategorie: Spike S1
Application Verdünnung: WB,1:2000 - 1:10000|IF/ICC,1:200 - 1:800|ELISA,1:1000 - 1:5000
Anwendungsbeschreibung: Cross-Reactivity: SARS-CoV-2
Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SARS-CoV-2 Spike S1 using SARS-CoV-2 Spike S1 mAb (A20022) at 1:10000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 5µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180 s.