SARS-CoV-2 Spike RBD Mouse mAb

Produktübersicht

• Artikelname: SARS-CoV-2 Spike RBD Mouse mAb
• Artikelnummer: ABB-A23771
• Hersteller Artikelnummer: A23771
• Alternativnummer: ABB-A23771-100UL, ABB-A23771-20UL
• Hersteller: ABclonal
• Wirt: Mouse
• Kategorie: Antikörper
• Applikation: DOT, ELISA, IF, WB
• Spezies Reaktivität: Virus
• Immunogen: Recombinant protein (or fragment).This information is considered to be commercially sensitive.
• Alternative Synonym: sars-cov-2, spike glycoprotein, SARS-CoV-2 Spike RBD

Produktbeschreibung

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The spike glycoprotein is found on the outside of the virus particle and gives coronavirus viruses their crown-like appearance. This glycoprotein mediates attachment of the virus particle and entry into the host cell. S protein is an important target for vaccine development, antibody therapies and diagnostic antigen-based tests.

Produkteigenschaften

• NCBI: 43740568
• Reinheit: Affinity purification
• Target-Kategorie: Spike RBD

Anwendungsbeschreibung

• Application Verdünnung: WB,1:500 - 1:1000|DB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: SARS-CoV-2

Bilder

Western blot analysis of lysates from wild type (WT) and293T cells transfected withSARS-CoV-2 Spike RBD usingSARS-CoV-2 Spike RBD Mouse mAb (A23771) at1:500 dilution.
Secondary antibody:HRP Goat Anti-Mouse IgG (H+L)(AS003) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time:10s.

Immobilized Recombinant SARS-CoV-2 Spike RBD Protein (RP01278LQ) at 1µg/mL (100µL/well) can bind SARS-CoV-2 Spike RBD mAb (A23771) with a linear range of 0.78-50ng/mL.