APC Rabbit IgG isotype control, Monoclonal

Artikelnummer: ABB-A24173
Artikelname: APC Rabbit IgG isotype control, Monoclonal
Artikelnummer: ABB-A24173
Hersteller Artikelnummer: A24173
Alternativnummer: ABB-A24173-100T,ABB-A24173-5X100T,ABB-A24173-2X100T
Hersteller: ABclonal
Wirt: Rabbit
Kategorie: Antikörper
Applikation: FC
Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation: APC
The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells, by non-specific interactions with cellular proteins, carbohydrates, and lipids, or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen.An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Klonalität: Monoclonal
Konzentration: FC
Reinheit: Affinity purification
Application Verdünnung: FC,5 µl per 10 6 cells in 100 µl volume
Anwendungsbeschreibung: Shipping: Ice Bag
Flow cytometry: 1X10 6 293F cells (negative control,left)and THP-1 cells (right) were surface-stained with APC Rabbit anti-Human CD209/DC-SIGN mAb (A23594,5 µl/Test,orange line) or APC Rabbit IgG isotype control (A24173,5 µl/Test,blue line). Non-fluorescently stained cells were used as blank control (red line).