EIF4A1/EIF4A2/EIF4A3 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: EIF4A1/EIF4A2/EIF4A3 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A24293
• Hersteller Artikelnummer: A24293
• Alternativnummer: ABB-A24293-100UL, ABB-A24293-20UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ELISA, IF, IHC-P, IP, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: DDX2A, EIF4A, EIF-4A, eIF4A-I, eIF-4A-I, DDX2B, EIF4F, BM-010, eIF4A-II, eIF-4A-II, Fal1, RCPS, DDX48, MUK34, NUK34, NMP265, eIF4AIII, eIF4A-III, eIF-4A-III, EIF4A1/EIF4A2/EIF4A3

Produktbeschreibung

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Produkteigenschaften

• Molekulargewicht: 46kDa
• NCBI: 1973
• UniProt: P60842
• Reinheit: Affinity purification
• Target-Kategorie: EIF4A1/EIF4A2/EIF4A3

Anwendungsbeschreibung

• Application Verdünnung: WB,1:20000 - 1:80000|IHC-P,1:200 - 1:2000|IF/ICC,1:200 - 1:4000|IP,0.5µg-4µg antibody for 200µg-600µg extracts of whole cells|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat, ResearchArea: Epigenetics and Nuclear Signaling,DNA / RNA,Translation,Regulation.

Bilder

Western blot analysis of various lysates, using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at 1:20000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Immunohistochemistry analysis of paraffin-embedded Human kidney using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat lung using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.

Immunofluorescence analysis of C6 cells using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of MCF7 cells using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation of EIF4A1/EIF4A2/EIF4A3 from 300 µg extracts of MCF7 cells was performed using 2 µg of EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293). Rabbit IgG isotype control (AC005) was used to precipitate the Control IgG sample. IP samples were eluted with 1X reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using EIF4A1/EIF4A2/EIF4A3 Rabbit mAb (A24293) at a dilution of 1:40000.