Acetyl-Histone H3-K4 Rabbit mAb, Unconjugated

Produktübersicht

• Artikelname: Acetyl-Histone H3-K4 Rabbit mAb, Unconjugated
• Artikelnummer: ABB-A24340
• Hersteller Artikelnummer: A24340
• Alternativnummer: ABB-A24340-100UL,ABB-A24340-20UL,ABB-A24340-500UL,ABB-A24340-1000UL
• Hersteller: ABclonal
• Wirt: Rabbit
• Kategorie: Antikörper
• Applikation: ChIP, DOT, ELISA, IF, IP, WB
• Spezies Reaktivität: Human
• Immunogen: Synthetic peptide. This information is considered to be commercially sensitive.
• Konjugation: Unconjugated
• Alternative Synonym: H3.4, H3/g, H3FT, H3t, HIST3H3, Histone H3, HIST1H3A, Acetyl-Histone H3-K4

Produktbeschreibung

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Produkteigenschaften

• Klonalität: Monoclonal
• Klon-Bezeichnung: [ARC62521]
• Molekulargewicht: 15 kDa
• NCBI: 8290
• UniProt: Q16695
• Reinheit: Affinity purification
• Sequenz: ARTK(AC)QTARKSTGGK-C
• Target-Kategorie: Histone H3
• Antibody Type: Primary Antibody

Anwendungsbeschreibung

• Application Verdünnung: WB,1:1000 - 1:2000|DB,1:2000 - 1:5000|IF/ICC,1:50 - 1:200|IP,2µg-6µg antibody for 400µg-600µg extracts of whole cells|ChIP,5µg antibody for 5µg-10µg of Chromatin|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based
• Anwendungsbeschreibung: Cross-Reactivity: Human,Mouse,Rat,Other (Wide Range Predicted), ResearchArea: Signal Transduction,MAPK-Erk Signaling Pathway,MAPK-P38 Signaling Pathway,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Acetylation.

Bilder

Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37°C for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from C6 cells,using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. C6 cells were treated with TSA (1 uM) at 37°C for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37°C for 18 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25µg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at 1:3000 dilution.

Immunofluorescence analysis of C6 TSA and C6 cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa TSA and HeLa cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH-3T3 TSA and NIH-3T3 cells using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 600ug extracts of HeLa TSA cells using 5ug Acetyl-Histone H3-K4 Rabbit mAb (A24340). Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K4 Rabbit mAb (A24340) at a dilition of 1:1000.

Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb antibody (A24340) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.