Synthetic peptide. This information is considered to be commercially sensitive.
Konjugation:
Unconjugated
Alternative Synonym:
GNT3, GNT-III, MGAT3
There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified.
WB,1:1000 - 1:2000|ELISA,Recommended starting concentration is 1 µg/mL. Please optimize the concentration based on your specific assay requirements.
Anwendungsbeschreibung:
Cross-Reactivity: Mouse. ResearchArea: Tags and cell markers, subcellular markers, organelles, Golgi apparatus. Shipping: Ice Bag
Western blot analysis of various lysates using MGAT3 Rabbit mAb (A24347) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Negative control (NC): mouse skeletal muscle. Exposure time: 45s.
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